The 3000L-5000L Industrial Bioreactor is a core production equipment designed for microbial fermentation and large-scale cell culture. It caters to the industrial production needs of various fields such as biopharmaceuticals, vaccines, biological products, and industrial enzymes. Constructed with an all-stainless steel structure, the equipment is equipped with a precise control system and in-situ sterilization (SIP) function. It can achieve high-density culture and high product expression, meet GMP production standards, and provide customers with stable, efficient, and scalable biomanufacturing solutions.
Core Product Series
- Applications: High-density fermentation of E. coli, yeast, actinomycetes, and other microorganisms, which is used for the production of recombinant proteins, antibiotics, industrial enzyme preparations, etc.
- Key Features: The high-shear agitation system is adapted to the growth needs of microorganisms. It is equipped with precise DO and pH control, anti-foam and contamination-resistant design, and supports continuous fermentation and fed-batch fermentation processes.
- Applications: Large-scale cultivation of animal cells such as CHO cells, HEK293 cells, and hybridoma cells, which is used for the production of monoclonal antibodies, recombinant proteins, viral vaccines, and other biopharmaceuticals.
- Key Features: The low-shear agitation system protects cell viability. It enables precise control of temperature, pH, dissolved oxygen, and osmotic pressure, supports perfusion culture and fed-batch culture, and meets the strict compliance requirements of the biopharmaceutical industry.
Detailed Technical Parameters (3000L-5000L Universal Type)
|
Parameter |
3000L Specification |
5000L Specification |
|
Working Volume |
2100L–3000L |
3500L–5000L |
|
Material (wetted parts: 316L SS, outer shell: 304 SS) |
Wetted parts are made of 316L stainless steel, and the outer shell is made of 304 stainless steel |
Wetted parts are made of 316L stainless steel, and the outer shell is made of 304 stainless steel |
|
Agitation System (Microbial: Disc turbine impeller; Cell: Marine/boat impeller) |
Microbial type: Disc turbine impeller; Cell type: Marine/boat impeller |
Microbial type: Disc turbine impeller; Cell type: Marine/boat impeller |
|
Agitation Speed Range |
20–300 rpm |
20–250 rpm |
|
Aeration Mode (Bottom ring/sparge aeration, multi-gas mixing: air, O₂, N₂, CO₂) |
Bottom ring aeration/microporous aeration, supporting multi-gas mixing of air, oxygen, nitrogen, and carbon dioxide |
Bottom ring aeration/microporous aeration, supporting multi-gas mixing of air, oxygen, nitrogen, and carbon dioxide |
|
Controlled Parameters (Temperature, pH, DO, Agitation Speed, Foam, Level, Feed Rate) |
Temperature, pH, dissolved oxygen (DO), agitation speed, foam, liquid level, feeding rate |
Temperature, pH, dissolved oxygen (DO), agitation speed, foam, liquid level, feeding rate |
|
Sterilization Method (In-situ sterilization (SIP), 121–135℃) |
In-situ sterilization (SIP) with a sterilization temperature of 121–135℃ |
In-situ sterilization (SIP) with a sterilization temperature of 121–135℃ |
|
Pressure Rating (Jacket/Vessel Working Pressure: 0.3 MPa; Sterilization Pressure: 0.15 MPa) |
Jacket/vessel working pressure: 0.3 MPa; Sterilization pressure: 0.15 MPa |
Jacket/vessel working pressure: 0.3 MPa; Sterilization pressure: 0.15 MPa |
|
Control System (Siemens/PLC, local & remote monitoring, MES integration capable) |
Siemens/PLC control system, supporting local and remote monitoring, and can be connected to the factory's MES system |
Siemens/PLC control system, supporting local and remote monitoring, and can be connected to the factory's MES system |
|
Compliance (cGMP, FDA, EU GMP compliant, full validation documentation available) |
Complies with the relevant requirements of cGMP, FDA, and EU GMP, and provides complete validation documents |
Complies with the relevant requirements of cGMP, FDA, and EU GMP, and provides complete validation documents |
