5000L-100KL Microorganism Series Large-Scale Fermentation System is core equipment designed for industrial-scale biomanufacturing. It is fully compatible with large-scale production needs in multiple fields, including antibiotics, enzymes, amino acids, probiotics, bioenergy, and biochemicals. Based on DevoTion's mature technical accumulation in bioreactors, the system takes "precision control, high efficiency and stability, energy conservation and consumption reduction, and easy expansion" as its core advantages, realizing seamless connection from laboratory processes to industrial production and providing reliable large-scale fermentation solutions for global biomanufacturing enterprises.
Adopting a modular design concept, the system can flexibly configure functional units such as stirring, aeration, temperature control, feeding, and sterilization according to the characteristics of different microbial strains (aerobic/facultative anaerobic) and process requirements, balancing process adaptability and operational convenience. The core control system integrates real-time multi-parameter monitoring and intelligent regulation functions, ensuring the precise and stable control of key parameters such as temperature, pH value, dissolved oxygen (DO), stirring speed, and aeration rate during fermentation to maximize microbial metabolic efficiency and target product yield. Meanwhile, the system strictly complies with industrial production compliance requirements, with material selection and structural design meeting the hygiene standards of the biomanufacturing industry, supporting in-situ sterilization (SIP) and clean-in-place (CIP) to effectively reduce pollution risks and ensure production continuity and consistent product quality.
- Pharmaceutical Field: Large-scale fermentation production of biopharmaceutical products such as antibiotics, vaccines, and recombinant proteins;
- Industrial Enzymes: Efficient preparation of industrial enzymes including amylase, protease, and cellulase;
- Food & Feed: Production of food additives and feed raw materials such as amino acids, vitamins, and probiotics;
- Bioenergy & Environmental Protection: Biodiesel, biogas fermentation, and resource utilization of organic waste;
- Biochemicals: Synthesis of green chemicals such as organic acids, bioplastics, and bio-based materials.
Core Advantages
System Composition
Fermentation tank unit, stirring drive unit, aeration & gas mixing unit, temperature control unit, feeding & defoaming unit, process control unit, sterilization unit, and safety & auxiliary unit.
Technical Parameters
| Parameter Category | Detailed Specifications |
| Tank System | Working Volume: 5000L-100000L (customizable); Material: Tank body and head are made of SUS316L stainless steel, with the inner surface electrolytically polished to Ra≤0.4μm; Design Pressure: 0.3MPa (standard), with higher pressure levels available on request; Interface Configuration: Inoculation port, sampling valve, feeding port, exhaust port, temperature/DO/pH electrode interface, liquid level gauge interface, etc., all equipped with hygienic quick-connect fittings; Structural Design: Dead-angle-free welding, with an elliptical or conical head at the tank bottom for easy drainage and cleaning. |
| Stirring System | Drive Mode: Top-mounted zero-leakage magnetic drive with stepless speed regulation by a servo motor; Speed Range: 50-300rpm (adapted to tank volume, accuracy ±1rpm); Stirring Paddle Type: Double-layer six-flat blade turbine paddle (standard), with low-shear paddle and propeller paddle optional (for different fermentation systems); Motor Power: 11kW-550kW (matched to tank volume). |
| Aeration System | Gas Types: Air, O₂, N₂ (three paths as standard, CO₂ expandable); Aeration Rate Range: 0.1-2.0VVM (under standard conditions), automatically controlled by a mass flow meter with an accuracy of ±2%FS; Sterile Filtration: A 0.22μm hydrophobic sterile filter element is installed at the air inlet, which is high-temperature sterilizable and reusable; Gas Distribution: An annular gas distributor at the tank bottom improves gas-liquid contact efficiency. |
| Temperature Control System | Control Mode: Jacket heat exchange, PID automatic control, and circulating water heating/cooling; Temperature Control Range: 10℃-75℃ (lower range customizable); Temperature Control Accuracy: ±0.2℃, supporting segmented temperature program control; Heat Exchange Medium: Industrial soft water or ethylene glycol solution. |
| Process Control | pH Control: Range 2-12, accuracy ±0.1pH, PID automatic adjustment, with peristaltic pumps for acid/alkali feeding; Dissolved Oxygen (DO) Control: Range 0-150%, accuracy ±5%, adjusted through the linkage of stirring speed, aeration rate, and oxygen ratio; Defoaming Control: Capacitive defoaming probe for automatic foam height detection, with a peristaltic pump for precise defoamer addition; Feeding Control: 4-channel analog peristaltic pump as standard, supporting timed and quantitative feeding or parameter-linked feeding, expandable to 8 channels. |
| Sterilization System | Sterilization Mode: In-situ Sterilization (SIP) at 121℃ with an adjustable time of 0-60min; Sterilization Range: Tank body, stirring device, sensors, pipelines, and filtration system can be sterilized simultaneously; Control Mode: Fully automatic program control, real-time monitoring of the sterilization process, and automatic cooling after sterilization. |
| Control System | Display Interface: 10-15 inch color touch screen supporting Chinese-English switching; Data Management: Real-time recording of parameters such as temperature, DO, pH, speed, and aeration rate, generating trend curves, and supporting USB export and data traceability; Communication Interface: RS485/Ethernet interface supporting MODBUS protocol, connectable to the factory MES system; Remote Control: Optional DevoTion remote monitoring module for remote operation and fault alarm via mobile phone/PC. |
| Power & Energy Consumption | Power Supply: Three-phase 380VAC±10%, 50/60Hz; Energy Consumption: 0.5-2.5kWh/m³ (fluctuates according to the fermentation process and tank volume). |
