| module | Content |
| product name | Pilot-Scale Stainless Steel Fermenter (50L-1000L) |
| product model | 50/100/200/300/500/1000SS |
| Core positioning | A key pilot-scale equipment connecting R&D and production, focusing on process scale-up verification and data portability |
| Applicable Scenarios | Microbial fermentation pilot trials, process parameter optimization, small-batch sample production, and GMP compliance pre-verification |
The Pilot-Scale Stainless Steel Fermenter (50L-1000L) is a core equipment specifically developed for the bioprocess scale-up phase, aiming to build an efficient and reliable transition bridge from R&D to industrial production. Adopting food-grade 316L stainless steel for integrated molding, the tank body and head are seamlessly connected, featuring excellent corrosion resistance and thermal stability. It is suitable for fermentation systems with high viscosity, high oxygen consumption, and complex components. By optimizing the aspect ratio design (2.5-3:1) and high-efficiency agitation system, it significantly improves mass transfer and oxygen dissolution efficiency, ensuring that the process parameters during the pilot-scale phase can be accurately transferred to industrial production, thus greatly reducing technical transformation risks.
The equipment supports two sterilization modes: in-situ sterilization (SIP) and ex-situ sterilization. Combined with double mechanical seal or magnetic seal technology, it achieves sterile operation standards and effectively avoids contamination risks. Equipped with an intelligent control system, it can real-time monitor and precisely regulate key process parameters such as temperature, pH, dissolved oxygen (DO), aeration rate, and agitation speed. Data is automatically collected, stored, and traceable, fully meeting the process optimization and compliance requirements of the pilot-scale phase. Whether for traditional microbial products such as antibiotics, enzymes, and probiotics, or strain process verification in the field of synthetic biology, this equipment can provide stable and repeatable operational support, helping enterprises accelerate technology implementation and product industrialization.
Key Technical Parameters| Parameter Category | Parameter Description |
| Volume Range | 50L、100L、200L、300L、500L、1000L(Customizable Volume Available) |
| Tank Material | Tank Body/Head: 316L Stainless Steel; Tank Cover: 304 Stainless Steel; Inner Surface Roughness Ra≤0.8μm |
| Agitation System | Type: Top Mechanical Agitation / Bottom Magnetic Agitation (Optional); Impeller: Propeller/Turbine Type (Configurable on Demand) |
| Sealing Method | Double Mechanical Seal / Magnetic Seal (Sterile Design, No Leakage) |
| Aspect Ratio | 2.5-3:1 (Optimized for Mass Transfer and Oxygen Dissolution Efficiency) |
| Temperature Control | Control Range: 10-60℃; Precision: ±0.1℃; Control Mode: Jacket Heating/Cooling |
| pH Control | Measurement Range: 2.0-12.0; Precision: ±0.01pH; Automatic Acid/Alkali Dosing System (Dual-pump Linkage) |
| Dissolved Oxygen (DO) Control | Measurement Range: 0-200% air sat; Control Mode: Aeration Rate + Agitation Speed Linkage; DO Electrode In-situ Sterilization |
| Aeration System | Air Filtration Precision: 0.2μm; Aeration Range: 0.1-2.0 vvm; Flow Meter Precision Control |
| Pressure Control | Working Pressure: 0.01-0.1MPa; Precision: ±0.005MPa; Automatic Pressure Relief Protection |
| Sterilization Method | In-situ Sterilization (SIP) / Ex-situ Sterilization (Optional); Sterilization Temperature: 121℃; Sterilization Pressure: 0.12MPa |
| Control System | Intelligent PLC Control System; Supports Data Acquisition, Real-time Monitoring, Remote Operation, and DOE Experimental Design |
| Compliance | Meets Basic cGMP Requirements; FDA Certification Report for Materials Available |
| Dimensions (Example) | 50L: L×W×H≈800×600×1500mm; 1000L: L×W×H≈1500×1200×3500mm (Subject to Actual Product) |
| Power Requirement | 380V±10%, 50Hz; Power: 50L≈3kW, 1000L≈15kW (Adjustable According to Configuration) |
1. Accurate Data Migration: High consistency between pilot-scale and industrial parameters reduces process scale-up risks and shortens the transformation cycle
2. Safe and Reliable Material: 316L stainless steel is acid-base resistant and corrosion-resistant, suitable for complex fermentation systems, and meets food-grade standards
3. Efficient Mass Transfer & Oxygenation: Optimized aspect ratio and agitation design improve oxygen utilization rate and ensure efficient microbial growth
4. Enhanced Sterility Assurance: Dual sealing + compliant sterilization process results in low contamination risk, meeting the sterility requirements of pilot-scale trials
5. Intelligent & Easy Operation: The PLC control system supports multi-parameter linkage control and remote monitoring, with traceable data
6. Flexible Customization: Volume, agitation type, and sealing method can be configured on demand to adapt to different process requirements
7. Compliance Assurance: Meets cGMP standards, with authoritative certifications for materials and processes to meet regulatory requirements
