一、Core Working Principle

Based on the Raman scattering effect, a monochromatic laser is applied to the fermentation broth system through a dedicated probe. The unique "molecular fingerprint" formed by molecular inelastic scattering enables accurate identification of various substance characteristics in the system.
The probe collects scattering signals and transmits them to a spectral analysis device. A specialized detector records spectral information, which is converted into concentration data of key substances during fermentation using professional algorithm models, realizing real-time analysis.
An online closed-loop control system is established, with real-time data synchronized to the central control system to coordinately regulate key parameters such as feeding, temperature, pH and stirring during fermentation, achieving precise and intelligent control of the fermentation process. 

二、System Composition (For Fermentation)

Optical Probe: Made of hygienic-grade stainless steel, it features high temperature and pressure resistance as well as acid and alkali corrosion resistance. It can undergo steam sterilization, be directly embedded in fermentation tanks or conveying pipelines, and realize in-situ monitoring without sampling.
Laser Light Source: Adopts near-infrared laser, effectively reducing fluorescence interference from biological samples, adapting to high-turbidity fermentation broth environments, and ensuring stable signal collection.
Spectral Analyzer: Designed with high resolution and high sensitivity, it has fast scanning capability and can efficiently complete the collection and processing of spectral signals. 

Specialized Software：

• Real-time display of the concentration change trends of multiple components during fermentation, intuitively presenting the dynamics of key indicators such as substrates, products and thalli.
• Built-in special quantitative models for the fermentation industry, supporting custom modeling and automatic calibration to meet the needs of different fermentation processes.
• It has data storage and abnormal alarm functions, and can be seamlessly linked with existing fermentation control systems to achieve integrated management and control.
  

三、Core Applications in the Fermentation Industry

1. Real-time Monitoring of Key Parameters (Full Coverage)

Covers monitoring of nutrient concentrations such as carbon and nitrogen sources, detection of product and by-product contents, monitoring of bacterial status (density, biomass, metabolic activity), as well as real-time control of key process indicators including fermentation rate and endpoint.

2. Typical Application Scenarios

• Pilot/Lab Scale Stage: Rapidly establish fermentation kinetic models, optimize feeding strategies, shorten process development cycles, and improve process R&D efficiency.
• Large-scale Production: Realize full-process online quality control, effectively avoid batch fluctuations, and ensure stable product yield and purity.
• Special Fermentation Scenarios: Adaptable to various special fermentation processes including photosynthetic fermentation, high-density fermentation, continuous fermentation, and cell-free synthesis, providing customized monitoring solutions.
• Exception Warning: Captures abnormal conditions during fermentation in real time (such as contamination, substrate depletion, abnormal accumulation of by-products, etc.), issues timely alarms, reduces batch scrappage, and lowers production costs.