Enzymolysis Tank

Ⅰ、Core Structure (Fully Enclosed Vertical Three-Layer Design) 

• Inner Tank (Reaction Chamber): The core reaction zone, made of SUS304/SUS316L hygienic stainless steel with mirror polishing (Ra ≤ 0.4μm), no dead corners for cleaning, and no contamination. Volumes cover laboratory pilot test (5L～50L), pilot scale-up (50L～500L), and industrial production (1m³～100m³).
• Jacket layer (core temperature control component): Adopting forms such as full jacket and half-coil pipe, it can be heated by steam or cooled by cooling water/refrigerant to achieve precise regulation of reaction temperature.
• Insulation layer: Made of rock wool and polyurethane foam materials to reduce heat loss, stabilize reaction temperature, and provide protection against scalding.
• Stirring system: Equipped with various impeller types such as anchor, paddle and turbine types (suitable for different material viscosities), driven by a motor and reducer, with variable frequency speed regulation (10～150r/min), ensuring thorough mixing of materials and enzymes and avoiding uneven local concentration and temperature.
• Pipelines and interfaces: Equipped with quick-opening manholes, sight glasses, CIP cleaning balls (360° spraying), as well as standardized interfaces for feeding, discharging, sampling, enzyme addition and other purposes, matched with monitoring components such as thermometers, online pH meters and liquid level meters to meet production and monitoring requirements.
• Control system: PLC touch screen control, which can automatically adjust temperature, pH, stirring speed and reaction time. It supports automatic feeding, enzyme inactivation, discharging and data recording, and is suitable for automated production.

Ⅱ、Workflow (concise and efficient, adapted for industrialization)

1. Feeding: Put the substrates (proteins, starches, polysaccharides, etc.) and water into the tank to complete the preliminary mixing.
2. Tuning conditions: Control the temperature to the optimal temperature for the enzyme (35–60°C) via a jacket, adjust to the optimal pH (4.5–8.0) by adding acid/alkali, so as to provide the best environment for enzyme catalysis.
3. Enzymatic hydrolysis reaction: add the corresponding enzyme preparations (protease, amylase, etc.), stir and maintain the temperature, and allow the reaction to proceed continuously for several hours.
4. Termination reaction: Rapidly raise the temperature to 80–100°C by introducing steam to inactivate the enzyme, prevent excessive degradation of the substrate, and ensure product quality.
5. Discharging and Cleaning: Discharge the material after cooling to a safe temperature for downstream processing; subsequent automatic CIP cleaning and SIP in-place sterilization are performed to meet the requirements of continuous production.

Ⅲ、Core Strengths

• Precise temperature control: Temperature control accuracy ±0.5℃, stabilizes enzyme activity and ensures reaction consistency；
• Hygiene compliance: Fully enclosed design that meets GMP/FDA standards, eliminating contamination by miscellaneous bacteria；
• Easy to operate: automatic control, capable of linking upstream and downstream processes, reducing labor costs；
• Wide adaptability: The type of stirring paddle and reaction parameters can be adjusted according to material characteristics to meet the needs of various industries；
• Easy maintenance: CIP/SIP in-place cleaning and sterilization, no dead corners for cleaning, extending the service life of the equipment.